LOCUS       sequence-52499-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS1
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(538..558)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(583..600)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             611..633
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    648..672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(672..691)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(673..691)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(779..798)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcttgtagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcaagcct gcaggtcatt
      121 tagaagattt aagtaaatat ctgatttgag gaacctggga taagagtcct tttccataag
      181 agtatatatg actttttgtg cttcatcaaa acacgtgggg gttggtgctt taatcttctt
      241 ggctgtagat tctcgagtgc ggaagtcaat attgatttgt ttagcagcat ctgaatgcac
      301 aaatgcttta tatatctctt ctgctttaca gggcaaaaga tcagactctg ttttcttata
      361 gtcttcacaa gccagccaga actcaatatt ctcctcactg aattcagact ttaggaaact
      421 tccaaagaca ttttgaccag tttggttggc aagaagtttt tccagagatt gagaccattg
      481 cattacttca gcagcagaaa gtacatcctt ggacttggaa gatttcattc cagacatgga
      541 ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat gatgatggtg
      601 catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga attgttatcc
      661 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc gatctcgatc
      721 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      781 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
      841 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactggttgg ggcgccatct
      901 ccttgcatgc accattccct tgcggcggcg ggggctcacc ggcctcaacc tactactggg
//
