LOCUS       sequence-52500-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RGS1
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             89..109
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             607..624
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(677..695)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      691..738
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(862..881)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tccctctgaa taattttgtt tactttaaga aggagatata catatgcacc atcatcatca
       61 tcattcttct ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgtctggaat
      121 gaaatcttcc aagtccaagg atgtactttc tgctgctgaa gtaatgcaat ggtctcaatc
      181 tctggaaaaa cttcttgcca accaaactgg tcaaaatgtc tttggaagtt tcctaaagtc
      241 tgaattcagt gaggagaata ttgagttctg gctggcttgt gaagactata agaaaacaga
      301 gtctgatctt ttgccctgta aagcagaaga gatatataaa gcatttgtgc attcagatgc
      361 tgctaaacaa atcaatattg acttccgcac tcgagaatct acagccaaga agattaaagc
      421 accaaccccc acgtgttttg atgaagcaca aaaagtcata tatactctta tggaaaagga
      481 ctcttatccc aggttcctca aatcagatat ttacttaaat cttctaaatg acctgcaggc
      541 ttgacagtaa aggtggatac ggatccgaat tcgagctccg tcgacaagct tgcggccgca
      601 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      661 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      721 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
      781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      901 ccggcttttc cccggtcaag ctcctaaatc gggg
//
