LOCUS       sequence-52501-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CENTG1
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(637..657)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(682..699)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             710..732
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    747..771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(771..790)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(772..790)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(878..897)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggcttgtagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaannt
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcaagcca gaagttgttg
      121 ctgcttgcgc aaggtcacca ccttctgggc cacctcctgg aagacccgat ccacattgag
      181 cccataggtt gcacaagtct catagtagct gcagcgtttc atgtccgcgc acagagctct
      241 ggcacgagca tctcccacca cccgagggga ggaagcactg atcctgtctt gtgtccccac
      301 cagtgccaag gccaggcctc ctcgtccctc cccgcgaagg gaactcagct gcccatggag
      361 acggctcaca gcctggaaac tgttctcatc ctccaggctg aagacgaaga tcacagcatc
      421 tgcccagcct gagaacttgg catcaggtgc cccagcttcc tctcggatta gcaccagatg
      481 tgtctgtcca tccaccaaca tttctttctt gtactgctca ctctctgtct tctccagcac
      541 ctggtatgag ccagtcagga atcggtggat gagcgatgac ttcccactcc tggcatcgcc
      601 cagcacaccc aggcgcagtt caggaatgga gcgcatggat tggaagtaca ggttctcggt
      661 acccagatct acaccagaag aatgatgatg atgatggtgc atatgtatat ctccttctta
      721 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      781 agtcgtatta atttcgcggg atcgagatcg atctcgatcc tctacgccgg acgcatcgtg
      841 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
      901 g
//
