LOCUS       sequence-52503-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CENTG1
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             696..713
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(766..784)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      780..827
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atattttgtt tactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgcgctccat tcctgaactg
      121 cgcctgggtg tgctgggcga tgccaggagt gggaagtcat cgctcatcca ccgattcctg
      181 actggctcat accaggtgct ggagaagaca gagagtgagc agtacaagaa agaaatgttg
      241 gtggatggac agacacatct ggtgctaatc cgagaggaag ctggggcacc tgatgccaag
      301 ttctcaggct gggcagatgc tgtgatcttc gtcttcagcc tggaggatga gaacagtttc
      361 caggctgtga gccgtctcca tgggcagctg agttcccttc gcggggaggg acgaggaggc
      421 ctggccttgg cactggtggg gacacaagac aggatcagtg cttcctcccc tcgggtggtg
      481 ggagatgctc gtgccagagc tctgtgcgcg gacatgaaac gctgcagcta ctatgagact
      541 tgtgcaacct atgggctcaa tgtggatcgg gtcttccagg aggtggccca gaaggtggtg
      601 accttgcgca agcagcaaca acttctggct tgacagtaaa ggtggatacg gatccgaatt
      661 cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      721 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      781 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      841 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcggggtg
      901 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gct
//
