LOCUS       sequence-52504-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RND1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(691..711)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(736..753)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             764..786
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    801..825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(825..844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(826..844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcag acagggctct tctggggcag
      121 tgggctaggc ttgttcagac acagcatgga tgccgtccga aagatgctgt ggatgctctt
      181 ttctgaggtg aaagctgagc cttccaggta gatttctgca cccagctgct ttgctattgc
      241 acaaccctgc tcataggaga tgggcgcctg cttctggtgg gacagctcca tcagagtact
      301 caggtctgtt cgcaggtctg tcttgcagcc aatgagcaaa acgcgggtgc tgggacaata
      361 atctaggatt tctgtcctcc acttcttgag tgcgctgtcc actgtctctg gacggctgat
      421 gtcaaaacat agtaatactg catccgagtc gctgtagcag agtggacgga cattatcgta
      481 gtagggagat cctgaggtat cccagagact aagctccacc ctctgttcct ctgtctccaa
      541 acaggctgtg taattttcga acacggtggg cacataggtc tctggatagc aatccttcgc
      601 taacacttgc aacatcgcgg tcttcccaca ctgcacgtcc ccgaccagaa cgagcttaca
      661 tctggccacg actggctggg gggcccgcat ggattggaag tacaggttct cggtacccag
      721 atctacacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt cttaaagtta
      781 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt
      841 attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat cgtggccggc
      901 atcacc
//
