LOCUS       sequence-52505-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RND1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             761..778
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(831..849)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      845..890
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat catcatcatt
       61 cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcgg gccccccagc
      121 cagtcgtggc cagatgtaag ctcgttctgg tcggggacgt gcagtgtggg aagaccgcga
      181 tgttgcaagt gttagcgaag gattgctatc cagagaccta tgtgcccacc gtgttcgaaa
      241 attacacagc ctgtttggag acagaggaac agagggtgga gcttagtctc tgggatacct
      301 caggatctcc ctactacgat aatgtccgtc cactctgcta cagcgactcg gatgcagtat
      361 tactatgttt tgacatcagc cgtccagaga cagtggacag cgcactcaag aagtggagga
      421 cagaaatcct agattattgt cccagcaccc gcgttttgct cattggctgc aagacagacc
      481 tgcgaacaga cctgagtact ctgatggagc tgtcccacca gaagcaggcg cccatctcct
      541 atgagcaggg ttgtgcaata gcaaagcagc tgggtgcaga aatctacctg gaaggctcag
      601 ctttcacctc agaaaagagc atccacagca tctttcggac ggcatccatg ctgtgtctga
      661 acaagcctag cccactgccc cagaagagcc ctgtctgaca gtaaaggtgg atacggatcc
      721 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      781 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      841 ataactagca taaccccttg gggcctctaa cgggtcttga ggggtttttt tgctgaaagg
      901 aggaactata
//
