LOCUS       sequence-52506-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: YWHAE
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(799..819)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(844..861)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             872..894
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcat gaagtccata gtgtcagatt
      121 atcacgtaac aactgcatga taagtgtaga gtccttatag ctttcttcac tcagcgtatc
      181 cagttctgca attgcatcat caaaagctgc ttttgccaac ctgcaggcac ggtcagggga
      241 attaagaatt tcgtagtaga atacggaaaa attgagagca agacctaagc gaataggatg
      301 cgttggtgga agttctgtca ttgcaatatc actagcagct ttataagcca ctaggctgtt
      361 ctccgcagcc tccttcctgt cgtttcctgt ggcaaattct gccagatacc tgtggtagtc
      421 ccctttcatt ttataataga aaaccttgga ctcgccagtg ttagctgctg gaatgaggtg
      481 tttgtccagt acatccagaa tgtcacaaca gattaacttt agctcagtct caaccatttg
      541 ccgatattcc cgaatcattt ttagcttgtc ttctcctccc ttgttttctt ctttctgttc
      601 aatgctgctg attattctcc aggaggctct tctagctcca atcacattct tatatgcaac
      661 agataggagg tttctttctt caactgtcag ctccacatcc atccctgcta ctttcttcat
      721 tgactccacc atttcgtcgt atcgctcagc ctgctcggcc agcttcgcct ggtacaccag
      781 atcctctcga tcatccatgg attggaagta caggttctcg gtacccagat ctacaccaga
      841 agaatgatga tgatgatggt gcatatgtat atctccttct taaagttaaa caaaaattat
      901 ttctagagg
//
