LOCUS       sequence-52507-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: YWHAE
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..105
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             870..887
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatatacata tgcaccatca tcatcatcat
       61 tcttctggtg tagatctggg taccgagaac ctgtacttcc aatccatgga tgatcgagag
      121 gatctggtgt accaggcgaa gctggccgag caggctgagc gatacgacga aatggtggag
      181 tcaatgaaga aagtagcagg gatggatgtg gagctgacag ttgaagaaag aaacctccta
      241 tctgttgcat ataagaatgt gattggagct agaagagcct cctggagaat aatcagcagc
      301 attgaacaga aagaagaaaa caagggagga gaagacaagc taaaaatgat tcgggaatat
      361 cggcaaatgg ttgagactga gctaaagtta atctgttgtg acattctgga tgtactggac
      421 aaacacctca ttccagcagc taacactggc gagtccaagg ttttctatta taaaatgaaa
      481 ggggactacc acaggtatct ggcagaattt gccacaggaa acgacaggaa ggaggctgcg
      541 gagaacagcc tagtggctta taaagctgct agtgatattg caatgacaga acttccacca
      601 acgcatccta ttcgcttagg tcttgctctc aatttttccg tattctacta cgaaattctt
      661 aattcccctg accgtgcctg caggttggca aaagcagctt ttgatgatgc aattgcagaa
      721 ctggatacgc tgagtgaaga aagctataag gactctacac ttatcatgca gttgttacgt
      781 gataatctga cactatggac ttcatgacag taaaggtgga tacggatccg aattcgagct
      841 ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg
      901 ctaacaaagc ccgaaaagga agctgagttg gctgctgcca ccgctgagca a
//
