LOCUS       sequence-52508-e       12166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo JL, 
            Bonenfant D, Oppliger W, Jenoe P, Hall MN
  TITLE     Two TOR complexes, only one of which is rapamycin sensitive, have 
            distinct roles in cell growth control.
  JOURNAL   Mol Cell. 2002 Sep;10(3):457-68.
  PUBMED    12408816
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2002-09"; volume: "10(3)"; pages: "457-68"
COMMENT     Sequence Label: YCplac111-KOG1 (pAN58)
FEATURES             Location/Qualifiers
     source          1..12166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    61..82
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(97..102,103..120,121..127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    135..151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     140..162
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     159..175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     159..175
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1216..1245
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1252..1281
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6299..6316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6299..6315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6308..6330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7074..8236
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      7399..8236
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(8598..9692)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9705..10109)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(10129..10147)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10215..10319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10320..10388,10389..11180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10538..10557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11351..11939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11840..11859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12093..12110
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
       61 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      121 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      181 ttacgccaag cttgcatgcc tgcaggtcga ctctagaggc gtattccaat gacctgccgg
      241 actttctttt attaacaagt cgtaattgaa atatatcgtt atttaacccg cgaggaattc
      301 taaattcgtt taacaattca ctcacctcct cagtatcgag agtgcctgtt aataccacat
      361 caatatctac tattgtagtg gccataaact gcagtaaagt caacttatca ggataattgt
      421 tattccaatc aggtattacc gtacggttct catctttgat gtctttatga taagtcgcaa
      481 ccatcgtgca gtgaggagag gcgatctcag acaaaaacct tgttatgtat tctgtagaaa
      541 tatagttcaa tgaatcgata atgaccatgt gcttttttgc ctgtgtggca gtagcagccg
      601 gtagatatga tataatttgt ttcactaaat gtacaaaatc catctgcgta gcgtcaataa
      661 attgagtaca gtacgatggt ttattcactg tttcgaacga aatgtacaca attggatact
      721 catttccttt actcttgctc tggtggacga attcttggat gagcttgtat gaggtctggg
      781 caatagaatc taagcacaaa atgaaagggg agctttctgt gagcgataag attcttttca
      841 aaagaatgac agggttatgt gacgaactgg ccattatatc ttgttctacc tgtagcaata
      901 gcttgggttg gagctattaa aaagctctta agttatcatt ctccagaggt tataatggca
      961 tcgcttctta tatttggatt tttcaaggtg acccgcacta tgagatgata atcaaggcag
     1021 cattaatagt atcaaaaata ctttaataaa tcatcaatag gtagtacttt acataagtat
     1081 atagaggatt ttaaaacttg taaagttatt ttcgaatgca tttggtttgt agattccttt
     1141 gattacattt agcgaatcct attgcatgca gagaagggta aaagatacat aatgatcccc
     1201 gggttaatta acggtgaaca aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa
     1261 ttaatctcag aagaagactt gaacggactc gacggtgaac aaccggagat ttatggacct
     1321 cagccattaa agccactcaa tacggtgatg agacatgggt ttgaagagca ataccaaagt
     1381 gaccaacttt tacaatcact cgcgaatgat ttcatattct actttgatga taaacgacac
     1441 aaaactaatg gaaatccgat tcctgaagag gataaacaac gagatgtaaa tcgttattac
     1501 cagccgatta ccgattggaa gattatgaaa gatcgacaga agacagtaag tgcggccctt
     1561 ttattatgct tgaatttagg tgtcgatccc cctgatgtta tgaagacaca tccctgtgcc
     1621 agagttgaag catgggtaga cccattgaat ttccaggact cgaagaaagc aatagaacaa
     1681 attggtaaaa acttacaagc acagtacgaa actctgtctt tgagaacacg ctataaacaa
     1741 agcttggatc cttgtgtaga ggatgtcaaa cggttttgca actcgctaag aagaacttct
     1801 aaggaagata ggatactttt ccactacaac ggacatggtg tgcctaagcc tacaaaatca
     1861 ggtgaaattt gggttttcaa cagaggttat acacaatata ttccggtatc tttgtatgac
     1921 ctgcaaactt ggttaggtgc gccatgtata tttgtctatg attgcaacag tgctgaaaat
     1981 atattaatca actttcagaa gtttgtgcaa aagaggatta aagatgatga agagggaaat
     2041 catgatgttg ccgcgccatc accaacttca gcttatcaag attgtttcca attagcctct
     2101 tgcacttccg atgaactttt acttatgagt ccggaactac ccgcagattt gtttagttgt
     2161 tgtttgacct gtccaataga gattagcatc cgtatctttc tcatgcagtc acccttaaaa
     2221 gattctaaat ataaaatttt cttcgaaaat agtacttcaa atcaaccgtt tggtgatagt
     2281 aagaactcgt tcaagtccaa aatacccaac gtcaatatcc ctggaatgtt gtcggatagg
     2341 agaacgccgt tgggagaact aaattggatt tttacagcta taacggacac aattgcatgg
     2401 acatccctcc cccgaccact tttcaaaaaa ttattcagac atgatttaat gattgctgcg
     2461 cttttcagaa attttcttct agccaaaaga ataatgccgt ggtacaattg ccatcctgta
     2521 tctgatccag aattaccaga tagtataacc acacatccaa tgtggaaatc ttgggattta
     2581 gccatggatg aagtactgac taaaatagtc attgatctga aaaatgctcc tcccgctact
     2641 gccttagaga gtcagatgat tctacaacaa caagaaacct tacagaatgg tggcagctct
     2701 aagtcgaatg cccaagatac gaaagccgga agtattcaaa cacaatcgag atttgcagtg
     2761 gccaatttaa gtacaatgtc tctagtaaat aaccctgctc tccaatcaag aaaatctata
     2821 tctttgcaaa gctcacagca acaattacag cagcagcaac aacaacaaca acaatttaca
     2881 ggattttttg aacaaaattt gactgctttt gaattgtggt tgaaatatgc ttcaaatgtc
     2941 agacacccgc ctgagcagct tcctatcgtt ttacaggttc ttctttcgca agtgcatcgt
     3001 atacgtgcgt tggtactttt atccagattt ttagatttag gcccgtgggc tgtctacctg
     3061 tcactttcaa taggtatctt cccctacgtt ttgaagctgc tacaaagtcc agcacctgag
     3121 ttgaagccca tcctagtttt tatttgggcc cgtatcatgt caatagatta caaaaacacg
     3181 caaagtgaat taatcaaaga aaagggttat atgtattttg ttacagtgtt agtacctgat
     3241 tggggtgtta atggcatgtc agctacaaat ggctcagcca tgataaatag tgggaacccg
     3301 ttgacaatga ctgcatcgca gaatataaat ggtccatctt caagatatta tgaacgccag
     3361 caaggaaata gaacatccaa cttgggccac aataatcttc cattttatca ttctaatgat
     3421 accaccgatg aacaaaaagc tatggctgtc tttgttttgg catcattcgt tcgaaacttt
     3481 cctctggggc aaaaaaattg ttttagtttg gaactggtca acaagttgtg cttttatatt
     3541 gataattctg agatacccct actaagacag tggtgtgtta ttttattagg tttattgttt
     3601 gctgataacc ccttaaacag gtttgtatgt atgaatactg gtgctgttga aatattacta
     3661 aaatctttga aggaccctgt acctgaagtt agaacagctt ctatatttgc attgaagcat
     3721 ttcatatctg gatttcaaga tgcagaggta attttgagat tgcaacaaga atttgaagaa
     3781 cagtatcagc aattacattc gcaattacag catctccaaa atcaatccca tctacaacaa
     3841 caacagtccc agcaacagca gcagcatctt gagcagcagc agatgaaaat tgaaaagcag
     3901 attcgtcatt gtcaagttat gcaaaatcaa ctagaagtta ttgatttgag gaagttaaaa
     3961 agacaggaaa ttggcaactt gatctcaatc ttacctttaa tcaatgatgg ttcatcatta
     4021 gtccgtaagg aactagtcgt atatttttcg catattgtga gtcgttactc taactttttt
     4081 attgtagttg tattcaatga tttgttggaa gaaataaaac ttttagagaa aagtgatatc
     4141 aatactcgaa acacttccga taagtattca gttagtcagg gttccatttt ctacactgtt
     4201 tggaaatctc tcttgattct agccgaagat ccctttttag agaacaagga attatccaag
     4261 caggttatag attatatttt gcttgaatta agcgcccata aggaacttgg tggaccattt
     4321 gcagttatgg aaaaattttt gttgaaaaga agctcgaaag ctcaccaaac tggtaaattt
     4381 ggatttaatt cttcgcaggt tcaatttgta aaaagttcat tacgatcatt ctcaccaaat
     4441 gagagggtag ataataatgc attcaaaaaa gagcaacaac agcatgaccc aaagatatct
     4501 catccaatgc ggacatcttt agctaaactt ttccagagtc ttggttttag cgaatccaat
     4561 agtgactctg atactcaatc atcaaatact tcgatgaaat cgcatacctc aaaaaagggt
     4621 ccctcgggct tatatttatt gaatggcaat aataatattt atcctacagc tgagactcca
     4681 agattccgga agcatacaga acccttacaa ttgccattaa atagttcctt tttagactat
     4741 tcacgtgaat attttcaaga accacaaatg aaaaagcaag aggcggatga acctggcagt
     4801 gttgagtata atgctagatt gtggagacgt aacagaaatg aaactattat ccaggaaaca
     4861 cagggggaga aaaaattatc gatttatggg aattggtcca aaaaattgat tagtttaaat
     4921 aacaaaagtc aaccaaaact gatgaagttt gcccagtttg aagatcagct gattactgca
     4981 gatgatagaa gtaccattac agtttttgat tgggagaaag gaaagacttt gtccaaattc
     5041 tccaatggta ctccttttgg cacaaaagtt actgacctga aactcattaa cgaggatgac
     5101 tccgcccttt tattgacagg ttcttcagac ggtgttatca aaatatacag agactaccaa
     5161 gatgtcgaca cctttaaaat tgtatccgcc tggagaggct tgaccgatat gttattgaca
     5221 cctagatcca caggtttact aaccgaatgg ttacaaataa gaggatcatt gttaacaact
     5281 ggtgatgtta aggttatccg tgtctgggat gcgcatacag aaacggttga agtggacatt
     5341 cctgcaaaaa catcatcttt aattacatcc ttgacggctg atcagctagc aggtaatatc
     5401 ttcgtagcag ggtttgcaga tggctccctg agagtatatg atcgccgtct cgatccaaga
     5461 gattccatga tacgtcgctg gagagctgga aatgataagc agggagtttg gattaacaat
     5521 gttcatttac aaagaggtgg ttacagagaa ttggtcagtg gagctaccaa tggtgttgta
     5581 gagttatggg atataaggtc cgaagatccg gtagaatcgt ttgttgatca aaacgtaact
     5641 tctcagtacg gttcgcaaca aaaaccaacg acaatgacgt gcatgcaggt tcatgagcat
     5701 gctccgataa ttgcgacagg gactaaacaa ataaagatat ggacgacatc tggagatctt
     5761 ttgaattctt ttaaaaactc acataataat ggcgtaacaa gtacattagc cgctacggga
     5821 atcccaaagt cactgtccta ctcttccact tctgatgcat ttttatcttc gatggctttc
     5881 catcctcata gaatgatgat tgcggccacc aattctcacg attctattgt aaatatctac
     5941 aagtgtgaag acgagagaat tgattatttt tgatatgtgc catttctttt tttttctttc
     6001 atttagctgc aaaagagata tatatatata tatatatata atctattata taataaaagt
     6061 cgagtaaaaa attggtctga ttgtgaattt aacagctggt cgcaagttct ggaatttgtt
     6121 gttaattatt gtttcatgtt cgtataataa tacgatgttt agtaaagcag aaagttcaga
     6181 atattaaaag tctgttccaa tagtgtagcg gctatcacgt tgccttcaca cggcaaaggt
     6241 cccgagttcg atcctcggtt ggaacatttt aacatcgccg ggtaccgagc tcgaattcac
     6301 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6361 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6421 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     6481 cgcatctgtg cggtatttca caccgcatat atcgctgggc cattctcatg aagaatatct
     6541 tgaatttatt gtcatattac tagttggtgt ggaagtccat atatcggtga tcaatatagt
     6601 ggttgacatg ctggctagtc aacattgagc cttttgatca tgcaaatata ttacggtatt
     6661 ttacaatcaa atatcaaact taactattga ctttataact tatttaggtg gtaacattct
     6721 tataaaaaag aaaaaaatta ctgcaaaaca gtactagctt ttaacttgta tcctaggtta
     6781 tctatgctgt ctcaccatag agaatattac ctatttcaga atgtatgtcc atgattcgcc
     6841 gggtaaatac atataataca caaatctggc ttaataaagt ctataatata tctcataaag
     6901 aagtgctaaa ttggctagtg ctatatattt ttaagaaaat ttcttttgac taagtccata
     6961 tcgactttgt aaaagttcac tttagcatac atatattaca cgagccagaa attgtaactt
     7021 ttgcctaaaa tcacaaattg caaaatttaa ttgcttgcaa aaggtcacat gcttataatc
     7081 aactttttta aaaatttaaa atactttttt attttttatt tttaaacata aatgaaataa
     7141 tttatttatt gtttatgatt accgaaacat aaaacctgct caagaaaaag aaactgtttt
     7201 gtccttggaa aaaaagcact acctaggagc ggccaaaatg ccgaggcttt catagcttaa
     7261 actctttaca gaaaataggc attatagatc agttcgagtt ttcttattct tccttccggt
     7321 tttatcgtca cagttttaca gtaaataagt atcacctctt agagttcgat gataagctgt
     7381 caaacatgag aattaattcc acatgttaaa atagtgaagg agcatgttcg gcacacagtg
     7441 gaccgaacgt ggggtaagtg cactagggtc cggttaaacg gatctcgcat tgatgaggca
     7501 acgctaatta tcaacatata gattgttatc tatctgcatg aacacgaaat ctttacttga
     7561 cgacttgagg ctgatggtgt ttatgcaaag aaaccactgt gtttaatatg tgtcactgtt
     7621 tgatattact gtcagcgtag aagataatag taaaagcggt taataagtgt atttgagata
     7681 agtgtgataa agtttttaca gcgaaaagac gataaataca agaaaatgat tacgaggata
     7741 cggagagagg tatgtacatg tgtatttata tactaagctg ccggcggttg tttgcaagac
     7801 cgagaaaagg ctagcaagaa tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc
     7861 aacaagtttg attccattgc ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt
     7921 cctgtacaat caatcaaaaa gccaaatgat ttagcattat ctttacatct tgttatttta
     7981 cagattttat gtttagatct tttatgcttg cttttcaaaa ggcttgcagg caagtgcaca
     8041 aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat
     8101 ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac
     8161 caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta
     8221 acataaaatg taagctctcg gggctctctt gccttccaac ccagtcagaa atcgagttcc
     8281 aatccaaaag ttcacctgtc ccacctgctt ctgaatcaaa caagggaata aacgaatgag
     8341 gtttctgtga agctgcactg agtagtatgt tgcagtcttt tggaaatacg agtcttttaa
     8401 taactggcaa accgaggaac tcttggtatt cttgccacga ctcatctcca tgcagttgga
     8461 cgatcgatga taagctgtca aacatgagaa ttaattctac cctatgaaca tattccattt
     8521 tgtaatttcg tgtcgtttct attatgaatt tcatttataa agtttatgta caaatatcat
     8581 aaaaaaagag aatcttttta agcaaggatt ttcttaactt cttcggcgac agcatcaccg
     8641 acttcggtgg tactgttgga accacctaaa tcaccagttc tgatacctgc atccaaaacc
     8701 tttttaactg catcttcaat ggccttacct tcttcaggca agttcaatga caatttcaac
     8761 atcattgcag cagacaagat agtggcgata gggtcaacct tattctttgg caaatctgga
     8821 gcagaaccgt ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc
     8881 aaggacgcag atggcaacaa acccaaggaa cctgggataa cggaggcttc atcggagatg
     8941 atatcaccaa acatgttgct ggtgattata ataccattta ggtgggttgg gttcttaact
     9001 aggatcatgg cggcagaatc aatcaattga tgttgaacct tcaatgtagg aaattcgttc
     9061 ttgatggttt cctccacagt ttttctccat aatcttgaag aggccaaaac attagcttta
     9121 tccaaggacc aaataggcaa tggtggctca tgttgtaggg ccatgaaagc ggccattctt
     9181 gtgattcttt gcacttctgg aacggtgtat tgttcactat cccaagcgac accatcacca
     9241 tcgtcttcct ttctcttacc aaagtaaata cctcccacta attctctgac aacaacgaag
     9301 tcagtacctt tagcaaattg tggcttgatt ggagataagt ctaaaagaga gtcggatgca
     9361 aagttacatg gtcttaagtt ggcgtacaat tgaagttctt tacggatttt tagtaaacct
     9421 tgttcaggtc taacactacc tgtaccccat ttaggaccac ccacagcacc taacaaaacg
     9481 gcatcaacct tcttggaggc ttccagcgcc tcatctggaa gtgggacacc tgtagcatcg
     9541 atagcagcac caccaattaa atgattttcg aaatcgaact tgacattgga acgaacatca
     9601 gaaatagctt taagaacctt aatggcttcg gctgtgattt cttgaccaac gtggtcacct
     9661 ggcaaaacga cgatcttctt aggggcagac ataggggcag acattagaat ggtatatcct
     9721 tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
     9781 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
     9841 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
     9901 cctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga
     9961 cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct
    10021 gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca
    10081 tcttacgata cctgagtatt cccacagtta attcttgaag acgaaagggc ctcgtgatac
    10141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    10201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10561 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10861 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11461 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    11521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    12001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    12061 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    12121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattca
//
