LOCUS       sequence-52512-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GMPR
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atattttgtt taactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgccccgca tagatgcgga
      121 cctcaagctc gacttcaagg atgtcctgct ccgacctaag cggagcagcc tcaagagccg
      181 agccgaggtg gatcttgaac gcaccttcac gtttcgaaat tcaaagcaga cctactcagg
      241 gattcccatc atcgtggcca acatggacac tgtgggcacg tttgagatgg cagccgtgat
      301 gtcacagcac tccatgttta cagcaattca taagcattac tccctggatg actggaagct
      361 ctttgccaca aatcacccag aatgcctgca gaatgtagcc gtgagttcag gcagtgggca
      421 gaatgatctg gaaaagatga ccagcatcct ggaagctgtg ccacaggtta agtttatttg
      481 cctggatgtg gccaatgggt attcagaaca ttttgtggaa ttcgtgaaac ttgtccgtgc
      541 caaatttcct gaacacacca ttatggcagg gaacgtggtg acaggagaaa tggtagaaga
      601 gcttattctt tccggagcag atatcatcaa agtgggagtt ggaccaggtt ctgtgtgcac
      661 cacccgcacc aagacgggag tggggtaccc ccagctgagt gccgtcattg agtgtgccga
      721 ctctgcccat ggcctgaagg gccacatcat ctctgatgga ggctgtacgt gtccagggga
      781 tgtcgccaaa gcctttggag ctggagcaga ttttgtcatg ctgggaggaa tgttttcggg
      841 tcatacggag tgtgctggag aagtgattga gaggaacgga cggaagctca agctcttcta
      901 cgggatgagc tctgacacc
//
