LOCUS       sequence-52517-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CLK1
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgcacct gatctgtcag
      121 agtggagacg tactaagtgc aagatatgaa attgttgata ctttaggtga aggagctttt
      181 ggaaaagttg tggagtgcat cgatcataaa gcgggaggta gacatgtagc agtaaaaata
      241 gttaaaaatg tggatagata ctgtgaagct gctcgctcag aaatacaagt tctggaacat
      301 ctgaatacaa cagaccccaa cagtactttc cgctgtgtcc agatgttgga atggtttgag
      361 catcatggtc acatttgcat tgtttttgaa ctattgggac ttagtactta cgacttcatt
      421 aaagaaaatg gttttctacc atttcgactg gatcatatca gaaagatggc atatcagata
      481 tgcaagtctg tgaatttttt gcacagtaat aagttgactc acacagactt aaagcctgaa
      541 aacatcttat ttgtgcagtc tgactacaca gaggcgtata atcccaaaat aaaacgtgat
      601 gaacgcacct taataaatcc agatattaaa gttgtagact ttggtagtgc aacctatgat
      661 gacgaacatc acagtacatt ggtatctaca agacattata gagcacctga agttatttta
      721 gccctagggt ggtcccaacc atgtgatgtc tggagcatag gatgcattct tattgaatac
      781 tatcttgggt ttaccgtatt tccaacacac gatagtaagg agcatttagc aatgatggaa
      841 aggattcttg ga
//
