LOCUS       sequence-52522-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Lungu OI, Hallett RA, Choi EJ, Aiken MJ, Hahn KM, Kuhlman B
  TITLE     Designing photoswitchable peptides using the AsLOV2 domain.
  JOURNAL   Chem Biol. 2012 Apr 20;19(4):507-17.
  PUBMED    22520757
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2012-04-20"; volume: "19(4)"; pages: "507-17"
COMMENT     Sequence Label: pET21b-LOV-ipaA L514K L531E
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             540..557
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(610..628)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      624..671
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(795..814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaaataattt tgtttaactt taagaaggag atatacatat gcatcatcat catcatcatg
       61 gcagcctggc gaccaccctg gaacgtattg aaaagaactt tgtgattacc gatccgcgtc
      121 tgccggataa cccgattatt tttgcgagcg atagctttct gcagctgacc gaatatagcc
      181 gtgaagaaat tctgggccgt aactgccgtt ttctgcaggg cccggaaacc gatcgtgcga
      241 ccgtgcgtaa aattcgtgat gcgattgata accagaccga agtgaccgtg cagctgatta
      301 actataccaa aagcggcaaa aaattttgga acctgtttca tctgcagccg atgcgtgatc
      361 agaaaggcga tgtgcagtat tttattggcg tgcagaagga tggtaccgaa catgtgcgtg
      421 atgcggcgga acgtgaaggc gtgatggaaa tcaagaaaac cgcgaacaac attattaaag
      481 cggcgaaaga tgtgaccacc agcctgagca aagtgctgaa aaacattaac taactcgagc
      541 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      601 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      661 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      781 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      841 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      901 cctcgaccc
//
