LOCUS       sequence-52524-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: BDH2
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(831..851)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(876..893)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg caagcttgtc
       61 gacggagctc gaattcggat ccgtatccac ctttactgtc acagtgacca accgccgtca
      121 ataatcacgg gattgccggt cacgtacgcg gattcatcag atgccagata cacacacagc
      181 attgcgattt cttccgccgt cgcaaaacgg ccggttttct ggcgttttaa gaagtcgtta
      241 cgggcttctt cagggttacc gcgggcctga atgcgttcct gcagcgacgg ggtatcaacg
      301 gtacccgggc acacgcaatt gcaacgaatg ccctgctgga taaaatccgc ggccacagat
      361 ttggtcagac caatcactgc cgctttcgta gtgctgtaga cacaacgatt cacgacgcct
      421 tttaccgagc tcgccacact ggacatgttg ataatattgc cgcttttctg cgccagcatt
      481 tttggcagga aggctttgat catcagatac atggaacgaa cattcaggtt catactaaag
      541 tcccagtctt tttcttcaca gtccagcaca gtgccgtgat gaacaaagcc cgcaacgtta
      601 aacagaacat ctaaacgttc cacttcgttc gcgaactgat caatctgttt ttttttagtg
      661 acatccagca cacgggtctg aatacccgga tatttttcca gttcctgcag ttttgactcg
      721 ttaatatcgg tggcaataac tttcgcacct tcgcgcgcga acgctaatgc cgctgcctgg
      781 ccgatgccct gggctgccgc ggtcagaatg atgactttgc catccagcat ggattggaag
      841 tacaggttct cggtacccag atctacacca gaagaatgat gatgatgatg gtgcatatgt
      901 atatctcctt ctttaaagtt taaacaaa
//
