LOCUS       sequence-52527-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GMDS
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctctgaatat tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgc gtaacgtggc
      121 gctgattacc ggcattaccg gccaggatgg cagctatctg gcggaatttc tgctggaaaa
      181 aggctatgaa gtgcatggca ttgtgcgtcg tagcagcagc tttaacaccg gccgtattga
      241 acatctgtat aaaaacccgc aggcgcatat tgaaggcaac atgaaactgc attatggcga
      301 tctgaccgat agcacctgcc tggtgaaaat tatcaacgaa gtgaaaccga ccgaaattta
      361 taacctgggc gcgcagagcc atgtgaaaat tagctttgat ctggcggaat ataccgcgga
      421 tgtggatggc gtgggcaccc tgcgtctgct ggatgcggtg aaaacctgcg gcctgattaa
      481 cagcgtgaaa ttttatcagg cgagcaccag cgaactgtat ggcaaagtgc aggaaattcc
      541 gcagaaagaa accaccccgt tttatccgcg tagcccgtat ggcgcggcca aactgtatgc
      601 gtattggatt gtggtgaact ttcgtgaagc gtataacctg tttgcggtga acggcattct
      661 gtttaaccat gaaagcccgc gtcgtggcgc gaactttgtg acccgtaaaa ttagccgtag
      721 cgtggcgaaa atttatctgg gccagctgga atgctttagc ctgggcaacc tggatgcgaa
      781 acgtgattgg ggccatgcga aagattatgt ggaagcgatg tggctgatgc tgcagaacga
      841 tgaaccggaa gattttgtga ttgcgaccgg cgaagtgcat agcgtgcgtg aatttgtgga
//
