LOCUS       sequence-52528-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B10
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(853..873)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(898..915)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcaa ccatccagac gaatcacttc
      121 gccgttcaga aacgggtttt cgataatcgc ctgcaccaga tgcgcatatt ccgccggatc
      181 acccagacgg ctctgaaacg gcacctggct cgccagaaag ttgcacactt tttccggcag
      241 gctggtcagc agcggggtac caaacagacc cggcgcaatg gtcatcacac gaatgccaat
      301 cggcgccaga tcacgcgcaa tcggcagggt catacccaca ataccgcctt tgctcgcgct
      361 ataggccgcc tgacccacct gaccttcaaa ggccgccacg ctcgcggtat tgataatcac
      421 accacgctga cctccctgat ccggttcatt ctgacccatt tcgcccgcca ccagacgaat
      481 cacgttaaag gtgcccatca ggttcacatc cagcacacgc tgaaaatctt ccagggtatg
      541 ggtctggcct tttttcaggt tataggtttt gctcgccacc gcaatgcccg cgcaattcac
      601 cgccacatcc acacgaccaa atttgccttt cgccagggcc agggcggtct gcacatcttt
      661 ttcgctggtc acatccgccg gcgcaaacac gcaattgttg cccagttttt tcgcctgcgc
      721 ttcaccaccg ctgttcggca gatccagcag cacggcgctc gcaccctgac ccaccagacg
      781 ttccgcggtc gccagaccca gaccgctcgc accaccggta atcaccgcca ccagaccttt
      841 cacgctacgc atggattgga agtacaggtt ctcggtaccc agatctacac cagaagaatg
      901 atgatgatga tggtgcatat gtatatctc
//
