LOCUS       sequence-52532-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD11B1
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(877..897)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc gtatccacct ttactgtcat ttgttaataa aacgatccat
      121 gttatagctg gtgctataca gaaattccag aattttacgg cacgggttac gaatcagcag
      181 ggtggtccac aggctgctat cataatacac ttcttcctga cgcagcgcac cgcctttgat
      241 aatttccagc gcgcattctt ctttcggggc cgcctgcatg tgcacaatgc cgctcaccgc
      301 tttcatcgcg gtttcgatat caatcaggcc cagcacgcac agggtaatgc tcacgttcac
      361 acggctcacg ctatattctt tacgaatgct gctaaaaaag ccatccagcg caaatttgct
      421 cgcgctatag gccgccacca tcggatacgc cactttgccc gccaggctgc tcaccaccac
      481 aatgctaccg ttgctctgtt tcagcatcgg cagggcggcc acggtcagca ccacatagct
      541 cagaaagttc acttccatgc ttttacgcac atgatgaata tcatcatgaa acaggttcag
      601 gctggtgttg gtaatatggt tcagaatcag catatccaga ccgcccatca gtttgcccgc
      661 ctgcgccaca aactgttccg caaaggtcat atcttccatc gtgcccgcaa tataatgcgc
      721 gctggccgcg cccagttcca ggcaatggct caccactttc tgcagggttt ctttgctacg
      781 cgcggtcacc accacatgcg cgcccatttt cgccagatga tacgccattt cacggccaat
      841 gcctttgctc gcgccggtca caatcacttt tttcatggat tggaagtaca ggttctcggt
      901 acc
//
