LOCUS       sequence-52533-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD11B1
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaataatttt gtttacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatgaaaaa agtgattgtg
      121 accggcgcga gcaaaggcat tggccgtgaa atggcgtatc atctggcgaa aatgggcgcg
      181 catgtggtgg tgaccgcgcg tagcaaagaa accctgcaga aagtggtgag ccattgcctg
      241 gaactgggcg cggccagcgc gcattatatt gcgggcacga tggaagatat gacctttgcg
      301 gaacagtttg tggcgcaggc gggcaaactg atgggcggtc tggatatgct gattctgaac
      361 catattacca acaccagcct gaacctgttt catgatgata ttcatcatgt gcgtaaaagc
      421 atggaagtga actttctgag ctatgtggtg ctgaccgtgg ccgccctgcc gatgctgaaa
      481 cagagcaacg gtagcattgt ggtggtgagc agcctggcgg gcaaagtggc gtatccgatg
      541 gtggcggcct atagcgcgag caaatttgcg ctggatggct tttttagcag cattcgtaaa
      601 gaatatagcg tgagccgtgt gaacgtgagc attaccctgt gcgtgctggg cctgattgat
      661 atcgaaaccg cgatgaaagc ggtgagcggc attgtgcaca tgcaggcggc cccgaaagaa
      721 gaatgcgcgc tggaaattat caaaggcggt gcgctgcgtc aggaagaagt gtattatgat
      781 agcagcctgt ggaccaccct gctgattcgt aacccgtgcc gtaaaattct ggaatttctg
      841 tatagcacca gctataacat ggatcgtttt attaacaaat gacagtaaag gtg
//
