LOCUS       sequence-52534-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B4
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(517..528)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggcttgtagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcacgcgg tgctggtcgc
      121 acggctggta tggttcgcgc tcacaccgcc ttcgctatca attttgctca gcacttcgat
      181 aatgctgccg gtgctttcct gaatgctctg cggtttgctc gcgttttcaa aatcgcaaat
      241 ttttttccag ttcgctttca ccgcttccgg ggtcatcgga tggtttttct gacgcacaat
      301 cgcgcccagg gtacgttccc aacgcagttt gccaatccag cccgcgccca cttcaaacag
      361 accgccgttt tcttcgcagc tttcatggca cagccacagc accagcggcg ccacatattc
      421 cggtttcagc gcttccacca gatcttccgg catcacggtc tgggtcatac ggctgcccgc
      481 gttcggcgca atggtgttgc aatgaatgtt gcttttacgg ccttcaatcg ccaggctgtt
      541 cgccaggccc agcaggccca gtttggccgc gctatagttc gcctggccaa agttgccata
      601 aatgccgctc gcgctgctgg tcatgataat acggccatat ttctgttttt tcatgtgttc
      661 ccaggccgca cgggtcacct gaaagctgcc acgcagatgc acacgatgga taatatccca
      721 atcttcatcg ctaatacgcg caaagctacg atcacgcaga atgcccgcat tgttcaccac
      781 cacatcaata cggccaaacg catccagcgc ggttttcacc actttttcgc cttcttcc
//
