LOCUS       sequence-52535-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B4
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             654..665
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gaaattccct ctagaataat tttgtttaac tttaagaagg agatatacat atgcaccatc
       61 atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgg
      121 gcagcccgct gcgttttgat ggccgtgtgg tgctggtgac cggcgcgggc gcgggcctgg
      181 gccgtgcgta tgcgctggcg tttgcggaac gtggcgcgct ggtggtggtg aacgatctgg
      241 gcggtgattt taaaggcgtg ggcaaaggca gcctggcggc cgataaagtg gtggaagaaa
      301 ttcgtcgtcg tggcggtaaa gcggtggcga actatgatag cgtggaagaa ggcgaaaaag
      361 tggtgaaaac cgcgctggat gcgtttggcc gtattgatgt ggtggtgaac aatgcgggca
      421 ttctgcgtga tcgtagcttt gcgcgtatta gcgatgaaga ttgggatatt atccatcgtg
      481 tgcatctgcg tggcagcttt caggtgaccc gtgcggcctg ggaacacatg aaaaaacaga
      541 aatatggccg tattatcatg accagcagcg cgagcggcat ttatggcaac tttggccagg
      601 cgaactatag cgcggccaaa ctgggcctgc tgggcctggc gaacagcctg gcgattgaag
      661 gccgtaaaag caacattcat tgcaacacca ttgcgccgaa cgcgggcagc cgtatgaccc
      721 agaccgtgat gccggaagat ctggtggaag cgctgaaacc ggaatatgtg gcgccgctgg
      781 tgctgtggct gtgccatgaa agctgcgaag aaaacggcgg tctgtttgaa gtgggcgcgg
      841 gctggattgg caaactgcgt tgggaacgta
//
