LOCUS       sequence-52537-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DHRS11
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             23..45
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             98..118
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgaggaattc cctctagaat attttgttta actttaagaa ggagatatac atatgcacca
       61 tcatcatcat cattcttctg gtgtagatct gggtaccgag aacctgtact tccaatccat
      121 ggcgcgtccg ggcatggaac gttggcgtga tcgtctggcg ctggtgaccg gcgcgagcgg
      181 cggtattggt gcggccgtgg cgcgtgcgct ggtgcagcag ggcctgaaag tggtgggctg
      241 cgcgcgtacc gtgggcaaca ttgaagaact ggcggccgaa tgcaaaagcg cgggctatcc
      301 gggcaccctg attccgtatc gttgcgatct gagcaacgaa gaagatattc tgagcatgtt
      361 tagcgcgatt cgtagccagc atagcggcgt ggatatttgc attaacaatg cggcccatat
      421 ggcgcgtccg gataccctgc tgagcggcag caccagcggc tggaaagata tgtttaacgt
      481 gaacgtgctg gcgctgagca tttgcacccg tgaagcgtat cagagcatga aagaacgtaa
      541 cgtggatgat ggccatatta tcaacattaa cagcatgagc ggccatcgtg tgctgccgct
      601 gagcgtgacc catttttata gcgcgaccaa atatgcggtg accgcgctga ccgaaggcct
      661 gcgtcaggaa ctgcgtgaag cgcagaccca tattcgtgcg acctgcatta gcccgggcgt
      721 ggtggaaacc cagtttgcgt ttaaactgca tgataaagat ccggaaaaag cggccgcgac
      781 ctatgaacag atgaaatgcc tgaaaccgga agatgtggcg gaagcggtga tttatgtgct
      841 gagcaccccg gcgcattgac agtaaaggtg gatacggatc cgaattcgag ctccgtcgac
      901 aagcttgcgg ccgcactcga gcaccaccac cacaccactg gaaaatccgg ctgctaaaaa
      961 aacccgaa
//
