LOCUS       sequence-52539-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PECR
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgagaattcc ctctaaaata ttttgtttac tttaagaagg agatatacat atgcaccatc
       61 atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgg
      121 cgagctgggc gaaaggccgt agctatctgg cgccgggcct gctgcagggt caggtggcga
      181 ttgtgaccgg cggtgcgacc ggcattggca aagcgattgt gaaagaactg ctggaactgg
      241 gcagcaacgt ggtgattgcg agccgtaaac tggaacgtct gaaaagcgcg gccgatgaac
      301 tgcaggcgaa cctgccgccg accaaacagg cgcgtgtgat tccgattcag tgcaacattc
      361 gtaacgaaga agaagtgaac aatctggtga aaagcaccct ggataccttt ggcaaaatta
      421 actttctggt gaacaatggc ggtggccagt ttctgagccc ggcggaacat attagcagca
      481 aaggctggca tgcggtgctg gaaaccaacc tgaccggcac cttttatatg tgcaaagcgg
      541 tgtatagcag ctggatgaaa gaacatggcg gtagcattgt gaacattatc gtgccgacca
      601 aagcgggctt tccgctggcg gtgcatagcg gcgccgcgcg tgcgggcgtg tataacctga
      661 ccaaaagcct ggcgctggaa tgggcgtgca gcggcattcg tattaactgc gtggcgccgg
      721 gcgtgattta tagccagacc gcggtggaaa actatggcag ctggggccag agcttttttg
      781 aaggcagctt tcagaaaatt ccggcgaaac gtattggcgt gccggaagaa gtgagcagcg
      841 tggtgtgctt tctgctgagc ccggcggcca gctttattac cggccagagc gtggatgtgg
      901 atggcggtcg
//
