LOCUS       sequence-52541-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B11
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             54..71
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             96..116
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgagaattcc cctgaataat tttgtttaac tttaagaagg agatatacat atgcaccatc
       61 atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgc
      121 cgaaacgtcg taaaagcgtg accggcgaaa ttgtgctgat taccggcgcg ggtcatggca
      181 ttggccgtct gaccgcgtat gaatttgcga aactgaaaag caaactggtg ctgtgggata
      241 ttaacaaaca tggcctggaa gaaaccgcgg ccaaatgcaa aggcctgggc gcgaaagtgc
      301 atacctttgt ggtggattgc agcaaccgtg aagatattta tagcagcgcg aaaaaagtga
      361 aagcggaaat tggcgatgtg agcattctgg tgaacaatgc gggcgtggtg tataccagcg
      421 atctgtttgc gacccaggac ccgcagattg aaaaaacctt tgaagtgaac gtgctggcgc
      481 atttttggac caccaaagcg tttctgccgg cgatgaccaa aaacaatcat ggccatattg
      541 tgaccgtggc gagcgcggcc ggccatgtga gcgtgccgtt tctgctggcg tattgcagca
      601 gcaaatttgc ggccgtgggc tttcataaaa ccctgaccga tgaactggcg gccctgcaga
      661 ttaccggcgt gaaaaccacc tgcctgtgcc cgaactttgt gaacaccggc tttattaaaa
      721 acccgagcac cagcctgggc ccgaccctgg aaccggaaga agtggtgaac cgtctgatgc
      781 atggcattct gaccgaacag aaaatgattt ttattccgag cagcattgcg tttctgacca
      841 ccctggaacg tattctgccg gaatgacagt aaaggtggat acggaatccg aattcgagc
//
