LOCUS       sequence-52543-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: HSD17B14
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..46
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             99..119
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgaggaattc cctctagaat aattttgttt aactttaaga aggagatata catatgcacc
       61 atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac ttccaatcca
      121 tggcgaccgg cacccgttat gcgggtaaag tggtggtggt gaccggtggt ggtcgtggta
      181 ttggtgcggg cattgtgcgt gcgtttgtga acagcggcgc gcgtgtggtg atttgcgata
      241 aagatgaaag cggtggtcgt gcgctggaac aggaactgcc gggcgcggtg tttattctgt
      301 gcgatgtgac ccaggaagat gatgtgaaaa ccctggtgag cgaaaccatt cgtcgttttg
      361 gccgtctgga ttgcgtggtg aataatgcgg gtcatcatcc gccgccgcag cgtccggaag
      421 aaaccagcgc gcagggcttt cgtcagctgc tggaactgaa cctgctgggc acctataccc
      481 tgaccaaact ggcgctgccg tatctgcgta aaagccaggg caacgtgatt aacattagca
      541 gcctggtggg tgcgattggt caggcgcagg cggtgccgta tgtggcgacc aaaggtgcgg
      601 tgaccgcgat gaccaaagcg ctggcgctgg atgaaagccc gtatggtgtg cgtgtgaact
      661 gcattagccc gggcaacatt tggaccccgc tgtgggaaga actggcggcc ctgatgccgg
      721 acccgcgtgc gagcattcgt gaaggtatgc tggcgcagcc gctgggtcgt atgggtcagc
      781 cggcggaagt gggtgcggcc gcggtgtttc tggcgagcga agcgaacttt tgcaccggta
      841 ttgaactgct ggtgaccggc ggtgcggaac tgggctatgg ttgcaaagcg agccg
//
