LOCUS       sequence-52545-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPR
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             90..110
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccctctaaaa taattttgtt taactttaag aaggagatat acatatgcac catcatcatc
       61 atcattcttc tggtgtagat ctgggtaccg agaacctgta cttccaatcc atgctgggtc
      121 gtgcggtgtg cctgctgacc ggtgcgagcc gtggttttgg tcgtaccctg gcgccgctgc
      181 tggcgagcct gctgagcccg ggtagcgtgc tggtgctgag cgcgcgtaac gatgaagcgc
      241 tgcgtcagct ggaagcggaa ctgggtgcgg aacgtagcgg tctgcgtgtg gtgcgtgtgc
      301 cggcggatct gggtgcggaa gcgggtctgc agcagctgct gggtgcgctg cgtgaactgc
      361 cgcgtccgaa aggcctgcag cgtctgctgc tgattaataa tgcgggcagc ctgggcgatg
      421 tgagcaaagg ctttgtggat ctgagcgata gcacccaggt gaacaattat tgggcgctga
      481 acctgaccag catgctgtgc ctgaccagca gcgtgctgaa agcgtttccg gatagcccgg
      541 gcctgaaccg taccgtggtg aatattagca gcctgtgcgc gctgcagccg tttaaaggtt
      601 gggcgctgta ttgcgcgggc aaagcggccc gtgatatgct gtttcaggtg ctggcgctgg
      661 aagaaccgaa tgtgcgtgtg ctgaattatg cgccgggtcc gctggatacc gatatgcagc
      721 agctggcgcg tgaaaccagc gttgatccgg atatgcgtaa aggcctgcag gaactgaaag
      781 cgaaaggcaa actggtggat tgcaaagtga gcgcgcagaa actgctgagc ctgctggaaa
      841 aagatgaatt taaaagcggc gcgcatgtgg atttttatga taaatgacag taaaggtgga
//
