LOCUS       sequence-52560-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Kaykas A, Yang-Snyder J, Heroux M, Shah KV, Bouvier M, Moon RT
  TITLE     Mutant Frizzled 4 associated with vitreoretinopathy traps wild-type 
            Frizzled in the endoplasmic reticulum by oligomerization.
  JOURNAL   Nat Cell Biol. 2004 Jan . 6(1):52-8.
  PUBMED    14688793
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2004 Jan . 6(1):52-8."
COMMENT     Sequence Label: XE240 Fz1-ER-GFP
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     5'UTR           85..124
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     sig_peptide     161..238
                     /codon_start=1
                     /product="human growth hormone signal sequence"
                     /label=hGH signal sequence
                     /translation="MATGSRTSLLLAFGLLCLPWLQEGSA"
     CDS             245..565
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=DmrD
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
       61 ccgggaccga tccagcctcc gggggatctt ggtggcgtga aactcccgca cctcttcggc
      121 cagcgaattc agggtcctgt ggacagctca cctagctgca atggctacag gctcccggac
      181 gtccctgctc ctggcttttg gcctgctctg cctgccctgg cttcaagagg gcagtgcctc
      241 tagaggagtg caggtggaga ctatctcccc aggagacggg cgcaccttcc ccaagcgcgg
      301 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaaa tggattcctc
      361 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg
      421 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga
      481 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt
      541 cgatgtggag cttctaaaac tggaaactag aggagtgcag gtggagacta tctccccagg
      601 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct
      661 tgaagatgga aagaaaatgg attcctcccg ggacagaaac aagcccttta agtttatgct
      721 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca
      781 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc accca
//
