LOCUS       sequence-52563-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: NEK2
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             867..889
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    905..928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gcttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtatt aaagggttct
       61 caagaatttc ttcaacagaa ggtcgatggt aatcctttaa gtttaacatc ctcgtaataa
      121 tttcattcaa ttcatcagag taacggtatg gaattcgcct gaatttgcct tctctgattt
      181 tcccagcgag ttctttctgg ctaaaagctg taaatggagg cattaatgca cataactcat
      241 acagcaagca gcccaatgac cagatatctg atttctcatt gtaggacatg cgattcattt
      301 gttcaggaga catgtaataa ggtgtgccaa caaatgcttt tgcaaaactc gtgtcgtggt
      361 ttaatattct agctagccca aagtctccaa gcttgacgtt ttgcttgcca tccaggaaaa
      421 cattggctgg tttcagatcc cgatgcaata cggtatgacc accatcactt cgtctgtggc
      481 attccttcag ggccagagtc aactgagtca tcactcgaag aacaaactct tcatctaagt
      541 attgcctttc cttggttccc tttgtaatta cactagccag atcccctcct tcacaatatt
      601 ccattacaat gtacagtgtt gtattggtcc ggtcaataat ccgatcatag taacgaacga
      661 tgtttggatg tttcagttca cgaagcaaat tcacttcaga aacaagcatc tgtttctcag
      721 cttctgtcat ggagccatag tcaagttctt tccaaactaa tatcttgcca tcactcttcc
      781 tccggatctt ctggcagcgg ccgtaggagc ctgtgccaat ggtgtacaac acttcatagt
      841 cctcagcccg ggaaggcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
      901 agggggaatt gttatccgct cacaattc
//
