LOCUS       sequence-52572-e        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Shyu YJ, Liu H, Deng X, Hu CD
  TITLE     Identification of new fluorescent protein fragments for bimolecular 
            fluorescence complementation analysis under physiological 
            conditions.
  JOURNAL   Biotechniques. 2006 Jan . 40(1):61-6.
  PUBMED    16454041
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2006 Jan . 40(1):61-6."
COMMENT     Sequence Label: pBiFC-bJunVN173
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      331..340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(337..339,340..342,343..855)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(382..403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(643..662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agagctcgtt tagtgaaccg tcagaattga tctaccatgg actacaaaga cgatgacgac
       61 aagcttgcgg ccgcgaattc aaaggcggag aggaagcgca tgaggaaccg catcgctgcc
      121 tccaagtgcc ggaaaaggaa gctggagcgg atcgcccggc tagaggaaaa agtgaaaacc
      181 ttgaaagcgc aaaactccga gctggcgtcc acggccaaca tgctcaggga acaggtggca
      241 cagcttaaac agaaagtcat gaaccacgtt aacagtgggt gccaactcat gctaacgcag
      301 cagttgcaaa cgttttctag aagatccatc gccaccatgg tgagcaaggg cgaggagctg
      361 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      421 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
      481 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
      541 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      601 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      661 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      721 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      781 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
      841 cgccacaaca tcgagtaggg atcccgggtg gcatccctgt gacccctccc cagtgcctct
      901 cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg
      961 catcattttg tctgactagg tgtccttcta taatattatg gg
//
