LOCUS       sequence-52576-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Thoreen CC, Chantranupong L, Keys HR, Wang T, Gray NS, Sabatini DM
  TITLE     A unifying model for mTORC1-mediated regulation of mRNA translation.
  JOURNAL   Nature. 2012 May 2;485(7396):109-13. doi: 10.1038/nature11083.
  PUBMED    22552098
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-05-2- 2"; volume: "485"; issue: "7396"; pages:
            "109-13"
COMMENT     Sequence Label: pLJM60-Tia1
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        196..214
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     196..213
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     227..246
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             305..328
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga
       61 acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt
      121 ctataggccc acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg
      181 tatcatacac atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc
      241 acaggtgtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat tcaccatgga
      301 aatggactac aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga cgatggagga
      361 cgagatgccc aagactctat acgtcggtaa cctttctcga gatgtgacag aagctctcat
      421 cctccagctc tttagccaga ttggaccttg taaaaactgc aaaatgatta tggatacagc
      481 aggaaatgac ccatactgtt ttgtggagtt ccatgagcac cgtcatgcag ctgcagccct
      541 ggctgccatg aatgggcgga agataatggg taaggaagtg aaagtgaatt gggcaacaac
      601 ccctagcagt caaaagaagg atacgagcaa tcatttccat gtgtttgttg gtgacctcag
      661 tccagaaatc acaaccgaag acatcaaagc agcgtttgca ccatttggaa gaatttcaga
      721 tgcccgtgtg gtaaaagaca tggctaccgg gaagtctaag ggatatggct ttgtctcctt
      781 tttcaacaaa tgggatgcag aaaatgccat tcagcagatg ggtggccagt ggcttggtgg
      841 aagacaaatc agaactaact gggcaacccg aaagcctcca gctccaaaga gtacatatga
      901 gtcgaacacc aaacagctgt catatgatga
//
