LOCUS       sequence-52577-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTPN5
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(963..983)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ctctttgggc tttgtagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattcgg atccgtatcc acctttactg tcaggactgg
      121 tgggacagct gcttttcgta gaggctcatg acgtggtgca caaactggta ctgctcgcat
      181 gtctggatca tgccgcccct gtcctgacgg agctggcacg tggtcttcag gatgtccact
      241 acaccctcct gccgcagctg ctggcagcag atgctggtgg caatgaagca gccggtcctc
      301 ccaatccctg cactgcagtg gacgatgatg ggggcacagt ggggcccctc ctgctgggct
      361 gcctcctcca cctcccgcac caggtgcagg agtggggggg cccggtctgg ggtcttctgg
      421 tcgggccagg atgtgaacca gtaatgcttc aggcctcgct cctcagtccc actcttgagg
      481 gagatgagtc gcagccggta atcctccgtg tgaatgactt tctgcacagt gatctcaaca
      541 ccgtcgtacg ccacctgctc ctccggccaa tactcggtgc atttctcgtt catctcctcg
      601 atgttggtga tcatgacaat gatgggcgtg tgctcctgcc acaccatgcg ccagaagtcg
      661 gcgaccgtgc tgacgatggg tccctgagtg gcgatgtaca ccttctcctc cccaccatag
      721 ccccggatgt agttggcatt gatgtaggaa ctcagagggt cgtcagggtc tggtgaggtc
      781 agacacactc tgctgtgagg gttgggaagt atggttttgt accggttctt ccgcaccagc
      841 ccagggatgt cgtactcttt cggatccaca aagttcatgg ggatttcaaa gaattccgcc
      901 tgcagcagga aagggtccag ggccttttca tgaagctctt ctgcttggag gacacgggac
      961 atggattgga agtacaggtt ctcggta
//
