LOCUS       sequence-52661-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MST4
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcgaattccc tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatggcc
      121 cactcgccgg tggctgtcca agtgcctggg atgcagaata acatagctga tccagaagaa
      181 ctgttcacaa aattagagcg cattgggaaa ggctcatttg gggaagtttt caaaggaatt
      241 gataaccgta cccagcaagt cgttgctatt aaaatcatag accttgagga agccgaagat
      301 gaaatagaag acattcagca agaaataact gtcttgagtc aatgtgacag ctcatatgta
      361 acaaaatact atgggtcata tttaaagggg tctaaattat ggataataat ggaatacctg
      421 ggcggtggtt cagcactgga tcttcttcga gctggtccat ttgatgagtt ccagattgct
      481 accatgctaa aggaaatttt aaaaggtctg gactatctgc attcagaaaa gaaaattcac
      541 cgagacataa aagctgccaa tgtcttgctc tcagaacaag gagatgttaa acttgctgat
      601 tttggagttg ctggtcagct gacagataca cagattaaaa gaaatacctt tgtgggaact
      661 ccattttgga tggctcctga agttattcaa cagtcagctt atgactcaaa agctgacatt
      721 tggtcattgg gaattactgc tattgaacta gccaagggag agccacctaa ctccgatatg
      781 catccaatga gagttctgtt tcttattccc aaaaacaatc ctccaactct tgttggagac
      841 tttactaagt cttttaagga gtttattgat gcttgcctga acaaagatcc atcatttcgt
      901 cctacagcaa aagaacttct g
//
