LOCUS       sequence-52667-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GHRHR
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(379..399)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(424..441)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     complement(454..513)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(561..652)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(619..636)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        676..785
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
ORIGIN
        1 cctctagtac ttctcgacaa gcttgtcgag actgcaggct ctagattcga aancggccgc
       61 gggaattcga ttctcgaatt cggatcctat ccacctttac tgtcattcct cctcagccag
      121 cagctccaga ggcacagggc aggccacagg gtaaggtgga aagggctcag accagccagt
      181 gatagtacaa tcccgtttca cagcccctga ctctgagctg aagtgagaga agaaatccgg
      241 gcaggggagg gtgacccact cgccagagcc tgccgttggc cagcacagca gcccatccca
      301 ggtcgcaggg cagcccaggg tggtttgggg catctcctct gctgcttgta gacaggcact
      361 ctcatcctct ctcagcatgg attggaagta caggttctcg gtacccagat ctacaccaga
      421 agaatgatga tgatgatggt ggcccatggc ggccgcaaag gcagaatgcg ccgccgccgc
      481 caaaagcaca tataaaacaa tagcgcttac catgttttag atccgcgccc gatggtggga
      541 cggtatgaat aatccggaat atttataggt ttttttatta caaaactgtt acgaaaacag
      601 taaaatactt atttatttgc gagatggtta tcattttaat tatctccatg atctattaat
      661 attccggagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa
      721 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
      781 caatcactcg acgaagactt gatcacccg
//
