LOCUS       sequence-52704-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: FASN
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             86..106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tctagaatat tttgtttaac tttaagaagg agatatacat atgcaccatc atcatcatca
       61 ttcttctggt gtagatctgg gtaccgagaa cctgtacttc caatccatgc gtctgctgcg
      121 ggccagcgga cgcacccctg aggccgtgca gaagctgctg gagcagggcc tccggcacag
      181 ccaggacctg gctttcctga gcatgctgaa cgacatcgcg gctgtccccg ccaccgccat
      241 gcccttccgt ggctacgctg tgctgggtgg tgagcgcggt ggcccagagg tgcagcaggt
      301 gcccgctggc gagcgcccgc tctggttcat ctgctctggg atgggcacac agtggcgcgg
      361 gatggggctg agcctcatgc gcctggaccg cttccgagat tccatcctac gctccgatga
      421 ggctgtgaag ccattcggcc tgaaggtgtc acagctgctg ctgagcacag acgagagcac
      481 ctttgatgac atcgtccatt cgtttgtgag cctgactgcc atccagatag gcctcataga
      541 cctgctgagc tgcatggggc tgaggccaga tggcatcgtc ggccactccc tgggggaggt
      601 ggcctgtggc tacgccgacg gctgcctgtc ccaggaggag gccgtcctcg ctgcctactg
      661 gaggggacag tgcatcaaag aagcccatct cccgccgggc gccatggcag ccgtgggctt
      721 gtcctgggag gagtgtaaac agcgctgccc cccgggcgtg gtgcccgcct gccacaactc
      781 caaggacaca gtcaccatct cgggacctca ggccccggtg tttgagttcg tggagcagct
      841 gaggaaggag ggtgtgtttg ccaaggaggt gcggaccggc ggtatggcct tccactccta
      901 ct
//
