LOCUS       sequence-52706-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: GPX4
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             669..686
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(739..757)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      753..800
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gggaattccc tctgaatatt ttgtttactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgcgc
      121 tgtgcgcgct ccatgcacga gttttccgcc aaggacatcg acgggcacat ggttaacctg
      181 gacaagtacc ggggcttcgt gtgcatcgtc accaacgtgg cgtcgcaagg tggaaagacc
      241 gaagtaaact acactcagct cgtcgacctg cacgcccgat acgctgagtg tggtttgcgg
      301 atcctggcct tcccgtgtaa ccagttcggg aagcaggagc cagggagtaa cgaagagatc
      361 aaagagttcg ccgcgggcta caacgtcaaa ttcgatatgt tcagcaagat ctgcgtgaac
      421 ggggacgacg cccacccgct gtggaagtgg atgaagatcc aacccaaggg caagggcatc
      481 ctgggaaatg ccatcaagtg gaacttcaca aagttcctca tcgacaagaa cggctgcgtg
      541 gtgaagcgct acggacccat ggaggagccc ctggtgatag agaaggacct gccccactat
      601 ttctagcagt aaaggtggat acggatccga attcgagctc cgtcgacaag cttgcggccg
      661 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      721 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      781 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      841 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      901 acttgccagc gccctagcgc
//
