LOCUS       sequence-52710-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: RERG
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             685..702
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(755..773)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      769..816
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatattttgt ttactttaag aaggagatat acatatgcac catcatcatc atcattcttc
       61 tggtgtagat ctgggtaccg agaacctgta cttccaatcc atggctaaaa gtgcggaggt
      121 caaactggca atatttggga gagcaggcgt gggcaagtca gctcttgtag tgagatttct
      181 gaccaaacgg ttcatctggg aatatgatcc caccctcgaa tcaacctacc gacaccaagc
      241 aaccatcgat gatgaagttg tttccatgga gatactagac actgctggtc aggaagatac
      301 cattcagagg gaggggcaca tgcgatgggg ggaaggcttt gtgctggtct acgacattac
      361 tgaccgagga agttttgagg aagtgctgcc acttaagaac atcctagatg agatcaaaaa
      421 gcccaagaat gtgactctca tcttggttgg aaacaaagct gacttggacc actccaggca
      481 ggttagcaca gaagaaggag agaagctggc cacagaattg gcttgtgctt tttacgagtg
      541 ctctgcctgc actggagaag ggaacatcac agagatattc tatgaattgt gtcgagaggt
      601 gcgtcgccgg aggatggtgt gacagtaaag gtggatacgg atccgaattc gagctccgtc
      661 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      721 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      781 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      841 ttggcgaatg ggacgcgcct gtagcgccat aacgcgcg
//
