LOCUS       sequence-52713-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AK2
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     regulatory      241..250
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg
       61 gtaccgagaa cctgtacttc caatccatgg ctcccagcgt gccagcggca gaacccgagt
      121 atcctaaagg catccgggcc gtgctgctgg ggcctcccgg ggccggtaaa gggacccagg
      181 cacccagatt ggctgaaaac ttctgtgtct gccatttagc tactggggac atgctgaggg
      241 ccatggtggc ttctggctca gagctaggaa aaaagctgaa ggcaactatg gatgctggga
      301 aactggtgag tgatgaaatg gtagtggagc tcattgagaa gaatttggag acccccttgt
      361 gcaaaaatgg ttttcttctg gatggcttcc ctcggactgt gaggcaggca gaaatgctcg
      421 atgacctcat ggagaagagg aaagagaagc ttgattctgt gattgaattc agcatcccag
      481 actctctgct gatccgaaga atcacaggaa ggctgattca ccccaagagt ggccgttcct
      541 accacgagga gttcaaccct ccaaaagagc ccatgaaaga tgacatcacc ggggaaccct
      601 tgatccgtcg atcagatgat aatgaaaagg ccttgaaaat ccgcctgcaa gcctaccaca
      661 ctcaaaccac cccactcata gagtactaca ggaaacgggg gatccactcc gccatcgatg
      721 catcccagac ccccgatgtc gtgttcgcaa gcatcctagc agccttctcc aaagccacat
//
