LOCUS       sequence-52723-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: ACAA1
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gaatattttg tttaacttta agaaggagat atacatatgc accatcatca tcatcattct
       61 tctggtgtag atctgggtac cgagaacctg tacttccaat ccatggcccc gcaggcctcg
      121 gccgcggacg tggtggtggt gcacgggcgg cgcacggcca tctgccgggc gggccgcggc
      181 ggcttcaagg acaccacccc cgacgagctt ctctcggcag tcatgaccgc ggttctcaag
      241 gacgtgaatc tgaggccgga acagctgggg gacatctgtg tcggaaatgt gctgcagcct
      301 ggggccgggg caatcatggc ccgaatcgcc cagtttctga gtgacatccc ggagactgtg
      361 cctttgtcca ctgtcaatag acagtgttcg tcggggctac aggcagtggc cagcatagca
      421 ggtggcatca gaaatgggtc ttatgacatt ggcatggcct gtggggtgga gtccatgtcc
      481 ctggctgaca gagggaaccc tggaaatatt acttcgcgct tgatggagaa ggagaaggcc
      541 agagattgcc tgattcctat ggggataacc tctgagaatg tggctgagcg gtttggcatt
      601 tcacgggaga agcaggatac ctttgccctg gcttcccagc agaaggcagc aagagcccag
      661 agcaagggct gtttccaagc tgagattgtg cctgtgacca ccacggtcca tgatgacaag
      721 ggcaccaaga ggagcatcac tgtgacccag gatgagggta tccgccccag caccaccatg
      781 gagggcctgg ccaaactgaa gcctgccttc aagaaagatg gttctaccac agctg
//
