LOCUS       sequence-52726-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBEN1-SGC
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..165
                     /codon_start=1
                     /product="solubility-enhancement tag derived from T7 phage 
                     gene 10B"
                     /label=SET1
                     /translation="DPEEASVTSTEETLTPAQEAARTRAANKARKEAELAAATAEQ"
     CDS             172..282
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             313..333
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        349..794
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 gaaatatttt gtttacttta agaaggagat atacatatgg accccgaaga ggcgagtgtt
       61 acttcaacag aagaaacctt aacgccagca caggaggccg cacgcacccg cgctgctaac
      121 aaagcccgaa aggaagctga gttggctgct gccaccgctg aacaaactag tgacgagaag
      181 accaccggct ggcggggcgg ccacgtggtg gagggcctgg ccggcgagct ggagcagctg
      241 cgggccaggc tggagcacca ccctcagggc cagcgggagc cctccggcgg ctgcaagctg
      301 ggcctgggta ccgagaacct gtacttccaa tccatggaga ccgacgtcca catatacctg
      361 ccgttcacta ttatttagtg aaatgagata ttatgatatt ttctgaattg tgattaaaaa
      421 ggcaacttta tgcccatgca acagaaacta taaaaaatac agagaatgaa aagaaacaga
      481 tagatttttt agttctttag gcccgtagtc tgcaaatcct tttatgattt tctatcaaac
      541 aaaagaggaa aatagaccag ttgcaatcca aacgagagtc taatagaatg aggtcgaaaa
      601 gtaaatcgcg cgggtttgtt actgataaag caggcaagac ctaaaatgtg taaagggcaa
      661 agtgtatact ttggcgtcac cccttacata ttttaggtct ttttttattg tgcgtaacta
      721 acttgccatc ttcaaacagg agggctggaa gaagcagacc gctaacacag tacataaaaa
      781 aggagacatg aacgatgaac atcaaaaagt ttgcaaaaca agcaacagta ttaacctttt
      841 actaccgcac tgctgg
//
