LOCUS       sequence-52727-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00512
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(672..771)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(788..807)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(789..807)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(812..828)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(812..828)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
       61 tataatgcca actttgtaca aaaaagttgg caccatgcac tcgcccgccc agtcgcccat
      121 tttggatgtc accgagaccg tatcgatgca ctccgaactg gatgcggaac tggagtccaa
      181 gaacaatccg tcctactcca tttccattcc ccagaatctt cggctaaccg gtctgggctt
      241 tcccggcatg atcgctgcca agagatcgtc ggaaactcta cccgccttcg agtatatcgc
      301 accaccaagt cacgctctcc aggcactgga atttccgctg atggagctca atcgtgtggg
      361 cgtaattgga ggcggtatgt ttccgggttt tgtgcaccgt cgcgtccgcg gtgaaaagcg
      421 acccattccg gaggctcaaa aggatgccaa gtactttgag cggcgcaagc gaaacaacga
      481 ggcggccaag aagtccagag atgcgcgtaa aatccgcgag gataggatag cattccgagc
      541 agcgctgctg gagcaggaga actccattct gcgggcgcag gtcctggcgc tgcgagatga
      601 actgcaaacg gtgcgccagc ttctcggagc cacaacagcc ggcggcatgt tgtccatggc
      661 caggcaggtg ttgccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
      721 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
      781 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
//
