LOCUS       sequence-52731-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SGC Empty backbone for baculovirus expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pFB-Sec-NH
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     81..140
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     regulatory      141..150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             195..215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(245..278)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        293..738
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 ttcgtacagt tttgtataaa aaaacctata aatattccgg attattcata ccgtcccacc
       61 atcgggcgcg gatctaaaac atggtaagcg ctattgtttt atatgtgctt ttggcggcgg
      121 cggcgcattc tgcctttgcg gccgccatgg gccaccatca tcatcatcat tcttctggtg
      181 tagatctggg taccgagaac ctgtacttcc aatccataag ctagcttctc ctcctgaaag
      241 atccataact tcgtatagca tacattatac gaagttatgc ggccgcgacg tccacatata
      301 cctgccgttc actattattt agtgaaatga gatattatga tattttctga attgtgatta
      361 aaaaggcaac tttatgccca tgcaacagaa actataaaaa atacagagaa tgaaaagaaa
      421 cagatagatt ttttagttct ttaggcccgt agtctgcaaa tccttttatg attttctatc
      481 aaacaaaaga ggaaaataga ccagttgcaa tccaaacgag agtctaatag aatgaggtcg
      541 aaaagtaaat cgcgcgggtt tgttactgat aaagcaggca agacctaaaa tgtgtaaagg
      601 gcaaagtgta tactttggcg tcacccctta catattttag gtcttttttt attgtgcgta
      661 actaacttgc catcttcaaa caggagggct ggaagaagca gaccgctaac acagtacata
      721 aaaaaggaga catgaacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc
      781 tttactaccg cactgctggc aggaggcgca actcaatcgt ttgcgaaaga aacgaacaaa
      841 gcatataaga acatacgcat tcc
//
