LOCUS       sequence-52737-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MCAT
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..92
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tttaacttta agaaggagat atacatatgc accatcatca tcatcattct tctggtgtag
       61 atctgggtac cgagaacctg tacttccaat ccatgggcca gtgctccgtg ctgctcttcc
      121 cgggccaggg cagccaggtg gtgggcatgg gccgcggtct gctcaactac ccgcgcgtcc
      181 gcgaactcta cgccgccgcc cgccgcgtgc tgggctacga cctgctggaa ctgagcctgc
      241 acgggccgca ggagaccctg gaccgcaccg tgcactgtca gcccgcgatc ttcgtggcat
      301 cgctggccgc tgtcgagaaa ctacatcacc tgcagccctc ggtgattgag aactgtgttg
      361 ctgctgctgg attcagtgtg ggagagtttg cagccctagt gtttgccgga gccatggaat
      421 ttgctgaagg tttgtatgca gtgaaaatcc gagctgaggc catgcaggaa gcttcagaag
      481 ctgtccccag tgggatgctg tctgtcctcg gccagcctca gtccaagttc aacttcgcct
      541 gtttggaagc ccgggaacac tgcaagtctt taggcataga gaaccccgta tgtgaagtgt
      601 ccaactacct ctttccagat tgcagggtga tttcaggaca ccaagaggct ctacg
//
