LOCUS       sequence-52738-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky 
            GE, Ellisman MH, Ting AY
  TITLE     Engineered ascorbate peroxidase as a genetically encoded reporter 
            for electron microscopy.
  JOURNAL   Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375.
  PUBMED    23086203
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375."
COMMENT     Sequence Label: pcDNA3 soybean APEX (IMS)
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             310..351
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tagcgagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgcggcc gccaccatgt accggctcct gtcaagcgtg
      121 acagctcggg ctgcggccac cgcaggccca gcctgggacg gagggcggcg cggggcgcac
      181 aggcgaccgg gcctgcctgt gctgggcctt ggttgggccg gcggcctggg gctcgggctg
      241 gggctggctc tcggcgcgaa gctggtggtc gggctgcggg gcgccgtccc cattcagtcc
      301 agggatccag gcaagcccat ccccaacccc ctgctgggcc tggacagcac cggaaagtct
      361 tacccaactg tgagtgctga ttaccaggac gccgttgaga aggcgaagaa gaagctcaga
      421 ggcttcatcg ctgagaagag atgcgctcct ctaatgctcc gtttggcatt ccactctgct
      481 ggaacctttg acaagggcac gaagaccggt ggacccttcg gaaccatcaa gcaccctgcc
      541 gaactggctc acagcgctaa caacggtctt gacatcgctg ttaggctttt ggagccactc
      601 aaggcggagt tccctatttt gagctacgcc gatttctacc agttggctgg cgttgttgcc
      661 gttgaggtca cgggtggacc taaggttcca ttccaccctg gaagagagga caagcctgag
      721 ccaccaccag agggtcgctt gcccgatgcc actaagggtt ctgaccattt gagagatgtg
      781 tttggcaaag ctatggggct tactgaccaa gatatcgttg ctctatctgg gggtcacact
      841 attggagctg cacacaagga gcgttctgga tttgagggtc cctggacctc taatcctctt
      901 attttcgaca actcatactt cacgg
//
