LOCUS       sequence-52742-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AKR7A3
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..97
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
       61 tgtagatctg ggtaccgaga acctgtactt ccaatccatg gcccggccag ccacggtgct
      121 gggcgccatg gagatggggc gccgcatgga cgcgcccacc agcgccgcag tcacgcgcgc
      181 cttcctggag cgcggccaca ccgagataga cacggccttc gtgtacagcg agggccagtc
      241 cgagaccatc cttggcggcc tggggctccg gctgggcggc agcgactgca gagtgaaaat
      301 tgataccaag gccattccac tgtttgggaa ctccctgaag cctgacagtc tccggttcca
      361 gctggagacg tcactgaagc ggctgcagtg tccccgagtg gacctcttct acctgcatat
      421 gccagaccac agcaccccgg tggaagagac actgcgtgcc tgccaccagc tgcaccagga
      481 gggcaagttc gtggagcttg gcctctccaa ctatgcagcc tgggaagtgg ccgagatctg
      541 taccctctgc aagagcaacg gctggatcct gcccactgtc taccagggca tgtacaatgc
      601 catcacccgg caggtggaaa cggagctctt cccctgcctc aggcactttg gactgaggtt
      661 ctatgccttc aaccctctgg ctgggggcct gctgaccggc aagtacaagt
//
