LOCUS       sequence-52743-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PIM2
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gttgaccaag cctctacagg
      121 ggcctcccgc gccccccggg acccccacgc cgccgccagg aggcaaggat cgggaagcgt
      181 tcgaggccga gtatcgactc ggccccctcc tgggtaaggg gggctttggc accgtcttcg
      241 caggacaccg cctcacagat cgactccagg tggccatcaa agtgattccc cggaatcgtg
      301 tgctgggctg gtcccccttg tcagactcag tcacatgccc actcgaagtc gcactgctat
      361 ggaaagtggg tgcaggtggt gggcaccctg gcgtgatccg cctgcttgac tggtttgaga
      421 cacaggaggg cttcatgctg gtcctcgagc ggcctttgcc cgcccaggat ctctttgact
      481 atatcacaga gaagggccca ctgggtgaag gcccaagccg ctgcttcttt ggccaagtag
      541 tggcagccat ccagcactgc cattcccgtg gagttgtcca tcgtgacatc aaggatgaga
      601 acatcctgat agacctacgc cgtggctgtg ccaaactcat tgattttggt tctggtgccc
      661 tgcttcatga tgaaccctac actgactttg atgggacaag ggtgtacagc cccccagagt
      721 ggatctctcg acaccagtac catgcactcc cggccactgt ctggtcactg ggcatcctcc
      781 tctatgacat ggtgtg
//
