LOCUS       sequence-52747-e         348 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 348)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: MUT
FEATURES             Location/Qualifiers
     source          1..348
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(99..122)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(123..140)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(141..161)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt gtcgacggag ctcgaattcg gatcctcact tatcgtcgtc atccttgtaa
      121 tcatggtggt gatgatggtg cgattggaag tagaggttct ctgctacaga ttgctgcttc
      181 ttttccaaac acttctcaat atcatcaagc acctgaacgg cagcctttgg aattcgagtc
      241 ccaggaccaa atacattgga aacaccaact tcaaacagaa attcataatc ctgaggtggt
      301 atcacccctc cacacatgac aagaatatct ggccgtccaa gggagtta
//
