LOCUS       sequence-52763-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SPSB4
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..114
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             801..818
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(871..889)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 attcccctct agaataattt tgtttaactt taagaaggag atatacatat gcaccatcat
       61 catcatcatt cttctggtgt agatctgggt accgagaacc tgtacttcca atccatgggt
      121 gcagagcccg ggcggccggc gcggctggac cagctgttgg acatgccagc ggcggggctg
      181 gctgtgcagc tgcggcacgc gtggaacccc gaggaccgct cgctcaacgt cttcgtcaag
      241 gacgacgacc ggctcacctt ccaccggcac cccgtggccc agagcaccga cggcatccgc
      301 ggcaaggtgg gccacgcccg cggcctgcac gcctggcaga tcaactggcc ggctcggcag
      361 cgcggcaccc acgctgtagt tggtgtggcc acggcccgtg ctcccctgca ctccgtgggc
      421 tacacggcgc tggtaggcag tgacgccgag tcgtggggct gggacctggg ccgcagccgc
      481 ctctaccacg acggcaagaa ccagcccggc gtggcctacc cggcctttct ggggcccgac
      541 gaggcctttg cgctgcccga ctcgctgctc gtggtgctgg acatggatga gggcacactc
      601 agcttcatcg tggatggcca gtacctgggc gtggccttcc gaggtctcaa gggcaagaag
      661 ctgtacccgg tggtgagtgc cgtgtggggc cactgtgaag tcaccatgcg ctacatcaac
      721 ggccttgacc ccgagtgaca gtaaaggtgg atacggatcc gaattcgagc tccgtcgaca
      781 agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag
      841 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      901 ggc
//
