LOCUS       sequence-52764-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CAMK2D
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             144..161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             186..206
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgcaccatc atcatcatca ttcttctggt gtagatctgg
      181 gtaccgagaa cctgtacttc caatccatga cggacgagta tcagcttttc gaggagcttg
      241 gaaagggggc attctcagtg gtgagaagat gtatgaaaat tcctactgga caagaatatg
      301 ctgccaaaat tatcaacacc aaaaagcttt ctgctaggga tcatcagaaa ctagaaagag
      361 aagctagaat ctgccgtctt ttgaagcacc ctaatattgt gcgacttcat gatagcatat
      421 cagaagaggg ctttcactac ttggtgtttg atttagttac tggaggtgaa ctgtttgaag
      481 acatagtggc aagagaatac tacagtgaag ctgatgccag tcattgtata cagcagattc
      541 tagaaagtgt taatcattgt cacctaaatg gcatagttca cagggacctg aagcctgaga
      601 atttgctttt agctagcaaa tccaagggag cagctgtgaa attggcagac tttggcttag
      661 ccatagaagt tcaaggggac cagcaggcgt ggtttggttt tgctggcaca cctggatatc
      721 tttctccaga agttttacgt aaagatcctt atggaaagcc agtggatatg tgggcatgtg
      781 gtgtcattct ctatattcta cttgtggggt atccaccctt ctgggatgaa gaccaacaca
      841 gactctatca gcaga
//
