LOCUS       sequence-52767-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DYRK1A
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagagganc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatgag ctcccataag aaggaacgta aggtgtacaa
      241 cgatggttat gacgacgata attacgacta catcgttaag aacggcgaga agtggatgga
      301 tcgctatgaa attgactcgc tgatcggtaa aggctctttc ggtcaggtcg taaaagccta
      361 tgatcgtgtg gagcaagaat gggtcgcaat aaagatcatt aagaacaaga aggcgttctt
      421 gaaccaggca cagatcgagg tccgcctgtt ggaactgatg aataagcacg ataccgagat
      481 gaaatactat atcgtgcatc tgaaacgtca cttcatgttt cgcaatcacc tgtgtttggt
      541 attcgagatg ctgtcataca acctgtatga cctgttgcgt aatactaact tccgcggcgt
      601 tagtttgaat ctgacacgca aattcgccca acaaatgtgc accgctttgt tgtttctggc
      661 gactccagaa ttgagcatta ttcattgtga tctgaaacct gagaacatcc tgttgtgcaa
      721 cccgaaacgc tccgcaatca aaattgtcga ctttggttct tcatgtcagc tgggccagcg
      781 tatctaccaa tacattcaga gtcgcttcta tcgttcgccc gaagtgttgc tgggtatgcc
      841 atacgatttg gccatcgaca tgtggagcct g
//
