LOCUS       sequence-52768-e         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: SLK
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             194..214
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt cttctggtgt
      181 agatctgggt accgagaacc tgtacttcca atccatgaag cagtacgaac acgtgacgag
      241 ggacctgaac cccgaagact tttgggagat tataggagaa ctgggcgacg gagcctttgg
      301 gaaagtgtac aaggcccaga ataaagagac cagtgtttta gctgctgcaa aagtgattga
      361 cactaaatct gaagaagaac ttgaagatta catggtagag attgacatat tagcatcttg
      421 tgatcaccca aatatagtca agcttctaga tgccttctat tatgagaaca atctttggat
      481 cctcattgaa ttttgtgcag gtggagcagt agatgctgtg atgcttgaac ttgagagacc
      541 attaactgag tcccaaatac aagtagtttg caagcagact ttagatgcat tgaactactt
      601 acatgataat aagatcatcc acagagatct gaaggctggc aacattctct ttaccttaga
      661 tggagatatc aaattggcgg attttggagt atcagctaaa aacacgagga caattcaaag
      721 aagagattcc tttattggta caccatattg gatggctcct gaagtagtca tgtgtgaaac
      781 atctaaggac agaccctatg actacaaagc tgatgtttgg tccctgggta tcactttaat
      841 agaaatggct gagatagaac cacctcatca tgaattaaat ccaatg
//
