LOCUS       sequence-52769-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTPN11
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             143..160
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..205
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgcaccatca tcatcatcat tcttctggtg tagatctggg
      181 taccgagaac ctgtacttcc aatccatggc tgagaccaca gataaagtca aacaaggctt
      241 ttgggaagaa tttgagacac tacaacaaca ggagtgcaaa cttctctaca gccgaaaaga
      301 gggtcaaagg caagaaaaca aaaacaaaaa tagatataaa aacatcctgc cctttgatca
      361 taccagggtt gtcctacacg atggtgatcc caatgagcct gtttcagatt acatcaatgc
      421 aaatatcatc atgcctgaat ttgaaaccaa gtgcaacaat tcaaagccca aaaagagtta
      481 cattgccaca caaggctgcc tgcaaaacac ggtgaatgac ttttggcgga tggtgttcca
      541 agaaaactcc cgagtgattg tcatgacaac gaaagaagtg gagagaggaa agagtaaatg
      601 tgtcaaatac tggcctgatg agtatgctct aaaagaatat ggcgtcatgc gtgttaggaa
      661 cgtcaaagaa agcgccgctc atgactatac gctaagagaa cttaaacttt caaaggttgg
      721 acaagggaat acggagagaa cggtctggca ataccacttt cggacctggc cggaccacgg
      781 cgtgcccagc gaccctgggg gcgtgctgga cttcctggag gaggtgcacc ataagcagga
      841 gagcatcatg gatgcagggc cggtcgt
//
