LOCUS       sequence-52770-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PTPRK
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             141..158
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             183..203
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg caccatcatc atcatcattc ttctggtgta gatctgggta
      181 ccgagaacct gtacttccaa tccatgccag ccatcagggt agctgattta ctgcagcaca
      241 ttaatctcat gaagacatca gacagctatg ggttcaaaga ggaatatgag agcttttttg
      301 aaggacagtc agcatcttgg gatgtagcta aaaaagatca aaatagagca aaaaaccgat
      361 atggaaacat tatagcatat gatcactcca gagtgatttt gcaacccgta gaggatgatc
      421 cttcctcaga ttatattaat gccaactata ttgatggcta ccagagacca agtcattaca
      481 ttgcaaccca aggtcccgtt catgaaacag tgtatgattt ctggaggatg atttggcaag
      541 aacaatctgc ttgcattgtg atggttacaa atttagttga ggttggccgg gttaaatgct
      601 ataaatattg gcctgatgat actgaagttt atggtgactt caaagtaacg tgtgtagaaa
      661 tggaaccact tgctgaatat gtagttagga cattcaccct ggaaaggagg gggtacaatg
      721 aaatccgtga agttaaacag ttccatttca cgggctggcc tgaccatgga gtgccctacc
      781 atgctacagg gctgctttcc tttatccggc gagtcaagtt atcaaaccct cccagtgctg
      841 gccccatcgt tgtacattgc agtgctggtg ctggacgaac tggctgctac attgtgattg
      901 acatcatgct aga
//
